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The applications of fluorescence excitation extend far beyond biological research to include many other areas such as combustion research, medical diagnostics, environmental monitoring, and chemical/pharmaceutical process monitoring.
In these applications, the common interest is to measure the intensity of fluorescence from an analyte in a sample. The analyte (known or unknown) undergoes selective excitation by laser, atomic line, or a broadband lamp coupled with a monochromator. Detection then yields information about species and concentration. In recent times, the surge in availability of fluorescent markers — from fluorescent proteins to quantum dots and nanoshells — has dramatically increased the tool kits of researchers making fluorescence-based measurements.
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